Fig. 6.

Effect of sulfide on ΔΨ in the absence and presence of rotenone. A, top trace: effect of applying 30 and then 500 μM Na₂S on ΔΨ. A, bottom trace: mitochondrial membrane potential during the application of the same concentrations of Na₂S following the prior application of 1 μM rotenone, which caused a rapid depolarization of on ΔΨ. B: means ± SE hyperpolarization of ΔΨ by 30 and 500 μM Na₂S in the presence and absence of rotenone. Bars represent the peak amplitudes of the monophasic hyperpolarization of ΔΨ recorded in 30 μM Na₂S and of the 2 phases of hyperpolarization recorded in 500 μM Na₂S (see example in A) in the presence and absence of rotenone in groups of tissues (n = 15 and 9 for controls and rotenone-treated arteries exposed to 30 μM Na₂S, respectively; n = 6 for both groups exposed to 500 μM Na₂S). *P < 0.05, rotenone vs. control by unpaired t-test.