Fig. 6.

Representative experiment of application of AICAR to the apical membrane (AP) of shark rectal gland primary cell cultures inhibits short-circuit current (I_sc). This experiment was repeated 5 times. Transepithelial chloride transport was measured as I_sc input across voltage clamped, confluent monolayers of shark rectal gland primary cell cultures. Inhibition of chloride secretion is reflected as a decrease in I_sc. Mean inhibition after AP addition of 1 mM AICAR was 27 ± 5.0% (n = 5). BL, basolateral.