Fig. 5.

Altered adhesion and expression of integrins in ChECs. A: adhesion of ChECs to fibronectin, vitronectin, collagen I, and collagen IV was determined as described in materials and methods. PEDF^−/− ChECs adhered less to various extracellular matrix (ECM) proteins compared with PEDF^+/+ cells. B: expression of integrins in ChECs; α1, α2, α3, α4, α5, α7, αv, αvβ3, α5β1, β1, β2, β3, β4, β5, and β8 integrin expression was determined by FACS analysis as described in materials and methods. Note lack of α1 detection in PEDF^−/− ChECs. The representative mean fluorescence intensities are indicated for each graph. These experiments were repeated using two different isolations of ChECs with similar results (n ≥ 3).