Fig. 9.

PEDF expression restored capillary morphogenesis of PEDF^−/− ChECs. PEDF^−/− ChECs were infected with lenti virus-encoding human PEDF as described in materials and methods. A: expression of PEDF was quantified using PCR. Note expression of human PEDF in the transfected cells compared with the mouse PEDF in the PEDF^+/+ cells. The human primers do not work well with mouse PEDF. B: the expression of PEDF was also confirmed by Western blot analysis. C: the capillary morphogenesis of PEDF^−/− ChECs expressing human PEDF in Matrigel. Note the restored capillary morphogenesis and increased capillary network stability of PEDF^−/− ChECs expressing PEDF (**P < 0.01, ****P < 0.0001, n = 3; scale bar = 100 µm).