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Increased oxidative stress in PEDF−/− ChECs. The level of oxidative stress in ChECs was assessed by dihydroethidium (DHE) staining under basal (A) or challenged conditions (200 μM H2O2) (B). A significant increase in the level of ROS was detected under basal and challenged conditions in PEDF−/− ChECs compared with PEDF+/+ cells (****P < 0.0001, n = 3; scale bar = 200 µm).
