Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 23;10(2):13. doi: 10.1038/s41368-018-0016-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Agc1-CreER directed Cre recombination in temporomandibular joint (TMJ) chondrocytes. a Agc1-CreER^T2; ROSA^mT/mG mice were generated by breeding Agc1-CreER^T2 transgenic mice with ROSA^mT/mG reporter mice. TMJ samples were harvested from 1-month-old mice after they were injected with tamoxifen at the age of 2 weeks for 5 consecutive days. Histologic sections from ROSA^mT/mG mice (Cre⁻) and Agc1-CreER^T2; ROSA^mT/mG mice were analyzed using fluorescence microscopy. High efficiency of Cre recombination (white arrowheads: Agc1-CreER^T2 targeting cells) in the TMJ chondrocytes, including the superficial, middle, and deep layers of condylar chondrocytes was found in Agc1-CreER^T2; ROSA^mT/mG mice. b, c Immunohistochemical (IHC) analysis showed that β-catenin expression was significantly increased in chondrocytes of 3- and 6-month-old β-cat(ex3)^Agc1CreER mice. Red arrowheads indicate β-catenin-positive chondrocytes. d Quantitative analyses of β-catenin-positive chondrocytes. A significant increase in the numbers of β-catenin-positive cells was observed in β-cat(ex3)^Agc1CreER mice compared to Cre⁻ mice (**P < 0.01; values are expressed as mean ± standard erros; n = 5 per group)