Fig. 5.

Na⁺-K⁺-ATPase α1-protein expression in the ISOM of the kangaroo rat and the Sprague-Dawley rat, the latter with water ad libitum (control) or water restricted for 72 h (see methods). A: immunoblots with 10 μg of protein applied to each lane; each lane represents a different animal. Black lines denote where blot was cut for rearrangement of lanes. B: quantification of Na⁺-K⁺-ATPase α1-immunoblot normalized to β-actin; n = 5 (kangaroo rat) and n = 3 (Sprague-Dawley rats). Kangaroo rat and water-restricted Sprague-Dawley rat data are expressed relative to the mean of the control Sprague-Dawley rat, which was set to 1. Kangaroo rats were mixed gender. The statistical significance between sample means was determined with a one-way ANOVA and Tukey’s post hoc test. Mean values that share the same letter are not significantly different from each other.