Fig. 7.

Interscapular brown adipose tissue surgery (iBATx) does not promote browning of white subcutaneous adipose tissue (inguinal region) in normal chow- and Western diet-fed mice. A: subcutaneous adipose tissue (AT) morphology at 26 wk of age in hematoxylin-eosin-stained sections. Magnification ×20 with ×10 objective. B and C: quantification of adipocyte cell size and adipocyte cell size distribution. D: expression of browning and inflammatory genes in epididymal adipose tissue, represented as fold difference from normal chow-sham. E: protein level of browning and mitochondrial markers in subcutaneous adipose tissue as determined by Western blot analysis, represented as fold difference from normal chow-sham. F: representative Western blots, for which experimental conditions were optimized for within-tissue comparisons. Values are means ± SE; n = 9–10/group. ^DMain effect of diet (P < 0.05). ^iBATxMain effect of iBATx (P < 0.05).