Fig. 8.

Interscapular brown adipose tissue surgery (iBATx) does not promote browning of white epididymal adipose tissue in normal chow- and Western diet-fed mice. A: epididymal adipose tissue (AT) morphology at 26 wk of age in hematoxylin-eosin-stained sections. Magnification ×20 with ×10 objective. B: expression of browning and inflammatory genes in epididymal adipose tissue, represented as fold difference from normal chow-sham. C: protein level of browning and mitochondrial markers in epididymal adipose tissue as determined by Western blot analysis, represented as fold difference from normal chow-sham. D: representative Western blots, for which experimental conditions were optimized for within-tissue comparisons. Values are means ± SE; n = 8–10/group. ^DMain effect of diet (P < 0.05).