Table 2.

Analytical Ultracentrifugation Parameters of AFF Variants^a

variant	s value (S)	% total peak area	apparent molecular mass (kDa)	theoretical molecular mass (kDa)
AFF70	1.93	82	35.1	40.6
AFF70	3.02	13	68.0	81.3 (dimer)
AFF186	1.85	82	39.5	42.6
AFF186	2.68	13	68.8	85.2 (dimer)
AFF210	1.87	89	33.7	40.1
AFF210	2.71	6.2	58.8	80.1 (dimer)
AFF70 with ribose	1.89	87	38.2	40.8
AFF70 with ribose	2.99	11	76.5	81.5 (dimer)
AFF186 with ribose	1.85	88	38.5	42.7
AFF186 with ribose	2.74	10	69.3	85.5 (dimer)
AFF210 with ribose	1.87	90	33.3	40.2
AFF210 with ribose	2.79	4.4	60.6	80.4 (dimer)

^aThe protein concentration was 10 μM and the temperature 10 °C.