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. Author manuscript; available in PMC: 2018 May 24.
Published in final edited form as: Biochemistry. 2013 Jan 17;52(4):600–612. doi: 10.1021/bi301105u

Table 3.

Thermodynamic Parameters of Ribose Binding Determined by ITCa

variant Kd (nM) −ΔH (kcal/mol) n
N′60 21.3 ± 1.1 16.4 ± 0.25 0.79 ± 0.032
N′70 2740 ± 240 17.5 ± 0.15 0.85 ± 0.010
N′186 38.1 ± 0.40 17.5 ± 0.21 0.78 ± 0.021
N′210 21.6 ± 1.4 18.3 ± 0.15 0.68 ± 0.012
AFF60 no binding detected no binding detected no binding detected
AFF70 46.6 ± 5.5 18.4 ± 0.15 0.75 ± 0.012
AFF186 58.8 ± 0.17 22.5 ± 2.1 0.44 ± 0.035
AFF210 26.1 ± 1.5 18.9 ± 0.71 0.17 ± 0.010
a

Errors are the standard deviations of three measurements. The temperature was 37 °C.