Figure 2. Percent inhibition for each small molecule in the CHIKV nsP1 high-throughput pilot screen.

Recombinant CHIKV nsP1 and GTP-bodipy were complexed and added to assay wells, 10 µM of compound was added to each well, plates were incubated, and total polarization and fluorescence polarization were assessed. Relative percent inhibition (the degree to which each small molecule was able to prohibit nsP1 GTP-binding compared to DMSO control) was calculated for each compound. n=2