Fig. 3.

Capturing performance of the CTCs enrichment platform equipped with a wedge-shaped microchamber. a The fluorescent micrographs of BGC823 captured from DMEM and healthy blood sample. Three-color immunocytochemistry method based on CK, CD45, and nuclear staining was applied to identify and enumerate BGC823 (red arrow) from WBCs (green arrow). Scale bars are 10 μm; b the recovered number of BGC823 was validated from DMEM and healthy blood sample with different spiking level (25, 50, 100, 150, 200 cells/2 mL, respectively); c the capture efficiency of four tumor cells lines (BGC823, HCT116, PC3, SKBR3) spiked in DMEM and healthy blood samples. All experiments were performed under optimal condition. The error bar represents standard deviation from three repeats