Fig. 3.

Cell cycle progression in SH-SY5Y and SK-N-SH cells was detected by flow cytometry. a, b Cells were respectively transfected with the miR-1247 mimics, NC-mimics, miR-1247 inhibitor, or NC-inhibitor, respectively for 48 h. Then the cells were stained with PI and subjected to flow cytometry analysis. The percentage of cells in G0/G1 phase, S phase and G2/M phase was the average value of three repeated experiments. c The protein expression of CDK1 and Cyclin D1 were analyzed in SH-SY5Y and SK-N-SH cells infected with miR-1247 mimics, NC-mimics, miR-1247 inhibitor, or NC-inhibitor by Western blot. GAPDH was used as the endogenous control. *p < 0.05, ***p < 0.001 versus NC-mimics; ^##p < 0.01, ^###p < 0.001 versus NC-inhibitor