Fig. 4.

Apoptosis in SK-N-SH was detected by flow cytometry. a, b Cells were respectively transfected with the miR-1247 mimics, NC-mimics, miR-1247 inhibitor, or NC-inhibitor, respectively for 48 h. Then the cells were stained with Annexin V/PI and subjected to flow cytometry analysis. The percentage cells in early apoptosis (Annexin V+/PI −) and late apoptosis (Annexin V+/PI +) was the average value of three repeated experiments. c The protein expression of Caspase 3 and Bcl-2 were analyzed in SK-N-SH cells infected with miR-1247 mimics, NC-mimics, miR-1247 inhibitor, or NC-inhibitor by Western blot. GAPDH was used as the endogenous control. ^##p < 0.01 versus NC-inhibitor; ***p < 0.001 versus NC-mimics