Fig. 5.

ZNF346 was identified as a target gene of miR-1247 in NB cells. a The miR-1247 binding sites in the ZNF346 3′UTR predicted by bioinformatics analysis. b Co-transfection of a wild type (WT) or a mutant ZNF346 3′UTR with miR-1247 mimics into SH-SY5Y and SK-N-SH cells. Luciferase activities were measured by Dual-luciferase reporter assay. c Western blot showing ZNF346 protein expression levels in SH-SY5Y and SK-N-SH cells after transfection with NC-mimics, miR-1247 mimics, NC-inhibitor and miR-1247 inhibitor, respectively. GAPDH was used as the endogenous control. **p < 0.01 versus NC-mimics