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. 2018 May 23;19:388. doi: 10.1186/s12864-018-4755-1

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© The Author(s). 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — mir-977 mutant generation. a Schematic representation of TALEN-mediated mutant generation and screening. b mir-977 deficiency verification. mir-977 KO results from a 21 bp deletion in its mature (highlighted in yellow) sequence