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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Int Immunopharmacol. 2018 Feb 3;56:249–260. doi: 10.1016/j.intimp.2018.01.037

Fig. 4. Induction of Th differentiation.

Fig. 4

Fig. 4

Fig. 4

Fig. 4

The intracellular cytokine staining of splenocytes in immunized C57BL mice (n=4) was performed at 2 weeks after the 3rd immunization. The frequency of CD4T-bet+ cells (A), CD4+GATA-3+ cells (B) and CD4+CD25+FoxP3+ cells (C) are shown. Representative dot plots from one mouse (out of four mice) for CD4+T-bet+ cell (D), CD4+GATA-3+ cells (E), and CD4+CD25+FoxP3+ cells (F) are shown respectively. Data are shown as mean ± s.d. Statistically significant differences (p < 0.05) are indicated.