Fig. 1. miR-494 and miR-99b are induced in response to radiation.

a Kinetics and b dose response of primary miR-494 and miR-99b transcripts in HUVECs treated with the indicated dose of radiation. Kinetics was measured in response to 2 Gy. The dose response was evaluated at 1 h post radiation. c In situ hybridization of miR-494 on FFPE slides from PyMT-MMTV tumors irradiated with the indicated dose of radiation. Tumors were harvested 36 h post radiation. Bars depict mean + SEM of sections from 3 mice/group. miR-494 staining was normalized to the number of cells stained with DAPI. Scale bar represents 200 µm. d qRT-PCR of mature miR-494 from orthotopic PyMT tumor implants (n = 4 tumors/group) at 6 h post radiation. miR levels were normalized to U6 small RNA. Bars depict mean + SEM. *P < 0.05; two-tailed Student’s T-test