Fig. 4. MRN pathway is necessary for sprouting angiogenesis.

a Fibrin bead angiogenesis assay. HUVECs were transfected with the indicated siRNAs and assessed for their sprouting angiogenesis potential. The images show representative lectin-stained beads (green) for each condition. Bars depict mean + SEM of lectin area analyzed across at least 25 beads/group. *P < 0.05; two-tailed Student’s T-test. b Fibrin bead angiogenesis assay. HUVECs were transfected with the indicated miRs and assessed for their sprouting angiogenesis potential. The images show representative lectin-stained beads for each condition. Bars depict mean + SEM of lectin area analyzed across at least 25 beads/group. c CD31 staining in vivo. bFGF-containing Matrigel plugs were implanted subcutaneously in nude mice and treated with miR-NC or miR-494 in vascular-targeted 7C1 nanoparticles on days 5 and 6. Mice were sacrificed at day 7 and plugs were harvested for tissue sections. Angiogenesis was measured by staining sections with anti-CD31 (green) and DAPI (blue). Scale bar represents 200 µm. Quantification of CD31 area from at least 3 mice/group is shown. Bars show mean + SEM