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. 2018 May 24;8:8078. doi: 10.1038/s41598-018-26281-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cross-recognition of wt/dr proteins and peptides in RT gene-immunized mice. Mice were immunized as described in Figs 2 and 3. RT1.14 and RTwt proteins were used to stimulate splenocytes of mice immunized (either in the prime or prime-boost regimen) with RTwt-opt-in and RT1.14-opt-in encoding plasmids, respectively (a–c). RT205–220 wt/dr peptides (Table 1; d) were used to stimulate splenocytes of mice immunized (in the prime regimen) with the RTwt-opt-in encoding plasmid (d). Results of the Flurospot test detecting IFN-γ, IL-2, and dual IFN-γ/IL-2 production are presented as the average number of cells, registered as signal-forming units (sfu) per million splenocytes secreting cytokines, and the error bars represent the SD. All assays were performed in duplicate. **p < 0.01; p-values in the interval 0.05 to 0.1 indicate tendencies; p > 0.1 for comparison of responses to RT205–220 wt and RT205–220 dr (d). Statistical comparisons were performed using Mann-Whitney tests.