Table 1.

Immune recognition by RTwt and RT1.14 gene-immunized mice of the peptides encompassing regions of drug resistance conferring mutations detected by IFN-γ/IL-2 Fluorospot.

Peptide used to stimulate murine splenocytes*	Cytokine responses raised in mice after immunization with RT genes
	RTwt-opt-in			RT1.14-opt-in
	IFN-γ	IL-2	IFN-γ/IL-2	IFN-γ	IL-2	IFN-γ/IL-2
RT 199–216 wt	>100	50–100	50–100	<50	<50	<50
RT 199–216 dr	>100	>100	>100	<50	<50	<50
RT 202–210 wt	<50	<50	<50	<50	<50	<50
RT 202–210 dr	<50	<50	<50	<50	<50	<50
RT 205–220 wt	>100	>100	>100	<50	<50	<50
RT 205–220 dr	>100	>100	>100	<50	<50	<50
RT 207–215 wt	50–100	<50	<50	<50	<50	<50
RT 207–215 dr	50–100	50–100	<50	<50	<50	<50

Each entry represents the results of two or more IFN-γ/IL-2 Fluorospot tests performed after single or repeated (double-prime and prime-boost) RT gene immunizations: >100 – more than 100, 50–100–50 to 100, <50 - less than 50 spot-forming cells per million stimulated lymphocytes (splenocytes). *Peptide sequences are given in Table 1. Results of Fluorospot tests that are discordant with the results of the multi-parametric FACS assays (Fig. 9) are in bold type. For the description of the immunization experiment, see “Materials and methods” and legends to Figs 2 and 3.