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. 2018 May 24;9(6):626. doi: 10.1038/s41419-018-0682-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Immunoblots and histogram showing the levels of EEA1, Sec31A, and p115 in control NSC34 cells and in cells treated with nocodazole (N), rapamycin (R), or the sequential combination (N2R3). b Microphotographs showing LAMP1 (green) and CTSB (red) immunostaining and co-localization (merged images) in indicated conditions. Below, graph bars of immunofluorescent quatification for each marker. Scale bar = 15 μm. c Representative confocal images of LAMP1 (red) co-immunolocalization (merged) with EEA1 (magenta) and giantin (green). Insets are two-fold magnifications of regions in boxes. Note that LAMP1 and giantin co-localized in cells treated with nocodazole. Scale bar = 30 μm