Fig. 6. Effects of nocodazole and rapamycin administration in vivo.

a Left, Microphotographs of fluorescence Nissl stained MNs at the ventral horns of L4–L5 segments of the spinal cord of unlesioned rats treated with a single injection of nocodazole (N) or vehicle (Veh) taken at 7 and 21 days post injection. Right, Bar graphs of the average percentage of MNs at these spinal cord segments compared to control animals at different time-points. b Representative images of Nissl stained MNs and associated bar graphs of MN quantification at the ventral horns of L4–L5 segments of spinal cord from animals treated with vehicle (Veh) or rapamycin (R), previously (R pre) or posteriorly (R post) to RA lesion at 21 days post injury (dpi). Scale bar = 50 µm