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. 2018 May 24;9(6):624. doi: 10.1038/s41419-018-0640-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Macrophages were infected with the Mtb H3 and H37Rv strains. After 2 h, uninfected and infected cells were treated with an IL-1β receptor antagonist or left untreated. After 20 h, cells were incubated with E64d + PepA to inhibit lysosome activity or left untreated. Autophagy levels were analyzed by evaluating LC3 and p62 expression by immunoblotting. GAPDH levels were analyzed to verify protein amount loading (upper panels). LC3-II/GAPDH ratios were quantified by densitometric analysis using the ImageQuant software. Each point value represents the mean ± SD from three independent experiments (lower panel)