Fig. 3.

Effect of therapies on self-renewal of RARA- or PML/RARA-transformed progenitors. a Mouse primary progenitors were immortalized by RARA overexpression and propagated in methylcellulose cultures with the indicated treatments. Treated cells were then regrown without further drug exposure. Colony numbers and MGG stains are shown. Scale bar: 10μm. b Same as above with PML/RARA-transformed progenitors. Representative experiment of three independent replicates. c Effect of RA therapy on the abundance of pre-leukemic PML/RARA-expressing cells in vivo. Relative PML/RARA DNA abundance, as determined by qPCR comparing PML/RARA and CEBPA abundance, is indicated for each mouse before and after therapy. Treatment did not result in significant difference in PML/RARA abundance, as demonstrated by paired Student's t-test The mouse images in this figure were drawn by Dr. Lehmann-Che for use in this paper