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. 2018 May 24;9(6):621. doi: 10.1038/s41419-018-0691-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Downregulation of HSF1, mutp53, and Mdm2 in lapatinib-sensitive parental BT474, but not in lapatinib-resistant BT474R cells, treated with indicated lapatinib concentrations for 24 h (a). b Lapatinib (300 nM, 48 h) blocks ERBB2 activation (measured by phospho-ERBB2) and its downstream effector Hsp70 in murine lapatinib-sensitive (1251, 1252, 1253), but not in lapatinib-resistant 125R cells. Hsc70 served as a loading control. c Lapatinib (300 nM, 24 h) blocks HSF1 activation (measured by phospho-Ser326) in human lapatinib-sensitive BT474, but not in lapatinib-resistant BT474R cells after heat shock (42°C, 30 min). Cells were pre-treated with lapatinib followed by heat shock. Western blot analyses. GAPDH as a loading control