Table 1.
BeRhGC (light–dark) | BeGC | CaRhGC (light–dark) | CaGC | CaAC (E497K, C566D) | |
---|---|---|---|---|---|
Substrate | GTP | GTP | GTP | GTP | ATP |
Hill fit R2 | 0.98 | 0.99 | 0.96 | 0.98 | 0.97 |
KM (mM) | 0.92 ± 0.27 | 2.16 ± 0.30 | 6.1 ± 5.59 | 5.78 ± 2.04 | 6.09 ± 1.74 |
n | 1 ± 0.18 | 1.72 ± 0.12 | 1.13 ± 0.42 | 1.22 ± 0.08 | 1.29 ± 0.06 |
vmax (cNMP (µmol min−1 mgprotein−1)) | 1.82 ± 0.19 | 1.89 ± 0.18 | 11.64 ± 5.67 | 6.30 ± 1.54 | 5.64 ± 1.24 |
vmax (cNMP (µmol min−1 µmolprotein−1)) | 128.5 ± 13.4 | 40.67 ± 3.87 | 821.8 ± 400.3 | 135.59 ± 33.14 | 121.38 ± 26.68 |
kcat (min−1) | 64.3 | 20.34 | 410.9 | 67.80 | 60.69 |
kcat (s−1) | 1.1 | 0.34 | 6.85 | 1.13 | 1.01 |
kcat/KM (s−1 mM−1) | 1.2 | 0.16 | 1.12 | 0.20 | 0.17 |
Molecular weight (kDa) | 70.6 | 21.5 | 70.6 | 21.5 | 21.5 |
Illumination | 522 nm, 0.010 mW mm−2 | 522 nm, 0.010 mW mm−2 |
Values are mean ± sem