Skip to main content
. 2018 May 24;9:2046. doi: 10.1038/s41467-018-04428-w

Table 1.

Enzymatic parameters of purified RhGCs and truncated cyclases

BeRhGC (light–dark) BeGC CaRhGC (light–dark) CaGC CaAC (E497K, C566D)
Substrate GTP GTP GTP GTP ATP
Hill fit R2 0.98 0.99 0.96 0.98 0.97
KM (mM) 0.92 ± 0.27 2.16 ± 0.30 6.1 ± 5.59 5.78 ± 2.04 6.09 ± 1.74
n 1 ± 0.18 1.72 ± 0.12 1.13 ± 0.42 1.22 ± 0.08 1.29 ± 0.06
vmax (cNMP (µmol min−1 mgprotein−1)) 1.82 ± 0.19 1.89 ± 0.18 11.64 ± 5.67 6.30 ± 1.54 5.64 ± 1.24
vmax (cNMP (µmol min−1 µmolprotein−1)) 128.5 ± 13.4 40.67 ± 3.87 821.8 ± 400.3 135.59 ± 33.14 121.38 ± 26.68
kcat (min−1) 64.3 20.34 410.9 67.80 60.69
kcat (s−1) 1.1 0.34 6.85 1.13 1.01
kcat/KM (s−1 mM−1) 1.2 0.16 1.12 0.20 0.17
Molecular weight (kDa) 70.6 21.5 70.6 21.5 21.5
Illumination 522 nm, 0.010 mW mm−2 522 nm, 0.010 mW mm−2

Values are mean ± sem