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. 2018 May 24;50(5):67. doi: 10.1038/s12276-018-0096-z

Fig. 1. Generation and characterization of anti-EGFR × cotinine bispecific antibody (ERC6).

Fig. 1

a A tetravalent bispecific anti-EGFR × cotinine antibody in IgG-HC-scFv of the Morrison format. b Purified ERC6 was subjected to 4–12% (w/v) SDS-polyacrylamide gel electrophoresis. The bands were visualized by staining with Coomassie Brilliant Blue R. Lanes 1 and 2 were loaded with and without reducing agent, respectively. c Purified ERC6 was subjected to size-exclusion chromatography-high-performance liquid chromatography. d ERC6 was intravenously injected into Balb/c mice (n = 4), and blood samples were collected via the intraorbital vein. The circulating serum levels of ERC6 were determined via enzyme immunoassays. The results are shown as the mean ± SD acquired from experiments conducted in triplicate. e Thermogram of ERC6 by differential scanning calorimetry. EGFR epidermal growth factor receptor