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. Author manuscript; available in PMC: 2018 May 24.
Published in final edited form as: FASEB J. 2008 Jan 22;22(6):2023–2036. doi: 10.1096/fj.07-099697

Figure 3.

Figure 3

H2O2 induces activation of the Ca2+-responsive kinases, Pyk-2, and Src tyrosine kinase. A) T84 monolayers were preincubated with the Src kinase inhibitor, PP2 (20 μM; 30 min; n = 4) prior to addition of H2O2, and subsequent Isc responses to CCh (100 μM) were measured. Data are expressed as percentage inhibition of CCh-stimulated Isc response. B, C) H2O2 (500 μM)-induced phosphorylation of Src (Y416) (B; n=6) and Pyk-2 (Y881) (C; n=4), over time (0 –30 min) was determined by Western blot and densitometric analyses. Values are presented as means ± SE. *P<0.05; **P<0.01; ***P<0.001 vs. control (H2O2 + CCh in A; untreated cells in B, C).