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Dynamics of Pol II at Hsp70 loci in vivo. FRAP (Fluorescence recovery after photobleaching) was used to measure dynamics of Pol II (using an Rpb3-GFP [green fluorescent protein] fusion protein) after 10- or 60-min following an instantaneous HS. Nonphotobleached Pol II is rapidly recruited to loci after 10 min but more modestly recruited after 60 min. Time (in seconds) along the x axis represents time elapsed after photobleaching.
