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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: J Neural Eng. 2018 Jun 1;15(3):033001. doi: 10.1088/1741-2552/aa9dae

Figure 8. Longitudinal GCaMP expression and neuro-physiological variance in AAV-Syn-GCaMP6f mice post-insertion.

Figure 8

GCaMP and intrinsic optical imaging sensitive to changes in blood oxygenation levels (OIS-BOLD) were recorded post-implantation. a) Representative images of GCaMP fluorescence over time from one of the mice tested. No large changes in overall fluorescence or superficial injury is observed. The temporal coefficient of variance (COV) was computed as an indication of tissue activity level from GCaMP (b) and OIS-BOLD (c) signals. d,e) Average ROI GCaMP ΔF/F and OIS-BOLD ΔS/S at each time point. Decreases in the GCaMP and OIS-BOLD COV were observed at the implanted area. The change in GCaMP COV diminished relative to neighboring tissue 7 days post-implantation, while the changes in OIS-BOLD COV appeared indifferent from neighboring tissue 4 days post-implantation. The changes in OIS-BOLD suggest there was a transient decrease in blood oxygen supply post-implantation.