Table 1:

Global metrics averaged across the analyses of simulated samples from human gut, ocean, and soil

		Family		Genus
Software	Database	Recall	Miscalled	Recall	Miscalled	Sub-regiona	Mean DS	Bray-Curtis	Jaccard
MAPseq	Greengenes	88.3	2.4	58.9	2.4	V3-V4	0.434	0.282	0.440
MAPseq	NCBI	81.7	1.3	51.7	2.0	V3-V4	0.522	0.330	0.495
MAPseq	SILVA	67.2	0.7	46.5	1.0	V3-V4	0.482	0.373	0.540
mothur	RDP	85.4	3.2	50.5	5.0	V3-V4	0.419	0.356	0.523
mothur	SILVA	82.9	2.4	40.8	5.2	V3-V4	0.492	0.446	0.613
QIIME 2	Greengenes	93.2	1.6	69.2	3.4	V3-V4	0.367	0.210	0.342
QIIME 2	SILVA	93.6	1.9	69.0	4.3	V3-V4	0.331	0.211	0.348
QIIME	Greengenes	59.4	1.6	45.1	2.5	V4	0.585	0.394	0.564
QIIME	SILVA	66.4	2.1	57.5	6.5	V4	0.432	0.309	0.470

Values in bold denote the best score.

^aSub-region with the highest F-score, excluding V1-V2.