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. Author manuscript; available in PMC: 2018 Nov 9.
Published in final edited form as: Nature. 2018 May 9;557(7705):387–391. doi: 10.1038/s41586-018-0105-3

Extended Data Fig. 7. OSM-3SNAP motor containing the wild type stalk colocalised with QCC in an IFT-70(DYF-1)-dependent manner.

Extended Data Fig. 7

(a) Neither TCC nor QCC w/o DYF-1 displayed efficient colocalisation with the OSM-3SNAP motor. In the presence of the IFT-70(DYF-1) subunit on the other hand, the QCC efficiently colocalised with OSM-3SNAP (81±8%). (b) Consistently, OSM-3SNAP showed robust colocalisation (82±6%) with the IFT-70(DYF-1) subunit but not with IFT-52(OSM-6), IFT-88(OSM-5), and IFT-46(DYF-6) subunits. IFT-81 from TCC was fluorescently labeled with SNAP-tag and IFT-52(OSM-6) from QCC and QCC w/o DYF-1 were GFP-tagged, respectively. Data presented as means of n=3 independent experiments ± SD; source data are provided online. Scale bar: 3 µm. C. elegans nomenclature was used in the figure due to space limitations.