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. Author manuscript; available in PMC: 2018 Nov 9.
Published in final edited form as: Nature. 2018 May 9;557(7705):387–391. doi: 10.1038/s41586-018-0105-3

Figure 3. Colocalisation efficiency of the OSM-3G444EHalo motor with its IFT-B complex is solely dependent on the IFT-70(DYF-1) subunit.

Figure 3

(a) Neither TCC nor QCC w/o DYF-1 sub-complexes displayed efficient colocalisation with the OSM-3G444EHalo motor. In the presence of the IFT-70(DYF-1) subunit on the other hand, the QCC complex efficiently colocalised with OSM-3G444EHalo (85±5%). (b) Consistently, OSM-3G444EHalo failed to interact with the individual IFT-52(OSM-6), IFT-88(OSM-5), and IFT-46(DYF-6) subunits but showed robust colocalisation (80±3%) with the IFT-70(DYF-1) subunit. IFT-81 and IFT-52(OSM-6) were fluorescently labeled with SNAP- and GFP-tags, respectively. Values presented as means of n=3 independent experiments ± SD; source data are provided online. Scale bar: 3 µm.