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. Author manuscript; available in PMC: 2018 Nov 9.
Published in final edited form as: Nature. 2018 May 9;557(7705):387–391. doi: 10.1038/s41586-018-0105-3

Extended Data Fig. 4. Pairwise colocalisation efficiency of IFT-B subunits.

Extended Data Fig. 4

Pairs of differentially labeled subunits of the IFT-B sub-complexes were incubated and analysed for their colocalisation efficiency. The columns (bottom panels) represent the percentage of colocalised spots in the corresponding colocalised images (top panels). All assayed combinations of the labeled subunits displayed significant colocalisation efficiencies demonstrating that C-terminal functionalisation of the subunits does not interfere with their complex formation capabilities. Values presented as means of n=3 independent experiments ± SD; source data are provided online. Scale bar: 3µm. C. elegans nomenclature was used in the figure due to space limitations.