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. Author manuscript; available in PMC: 2018 May 24.
Published in final edited form as: Mol Cancer Res. 2017 May 16;15(8):998–1011. doi: 10.1158/1541-7786.MCR-16-0494

Figure 6.

Figure 6

The core promoter region of ZBTB18 is essential for promoter activity and is sensitive to DNA methylation. (A) Schematic representation of the ZBTB18 promoter regions cloned in the pGL3 luciferase reporter vector. (B) Analysis of ZBTB18 promoter constructs activity by dual luciferase assay. (C) In vitro methylation assay of ZBTB18 promoter 4. The plasmid DNA was methylated with SssI or HpaII methylase. (D) Control restriction digestion of the methylase reaction using the methylation sensitive (HpaII) and the methylation insensitive (McrBC) restriction enzymes.