Table 2.

List of key genes related to NFκB signaling targets differentially expressed in LPS/IFNγ-activated and thymoquinone-treated BV-2 microglial cells. The fold regulation change of LPS/IFNγ vs. Control, TQ + LPS/IFNγ vs. LPS/IFNγ, and TQ vs. Control. P-values (t-test) are listed in the table; (n = 3).

Gene symbol	Encoded protein	Genes upregulated in the LPS/IFNγ vs. control			Genes downregulated in the TQ + LPS/IFNγ vs. LPS/IFNγ			Function
		Direction	Fold regulation LPS-IFNγ/control	p-Value (T-test)	Direction	fold regulation TQ + LPS-IFNγ/LPS-IFNγ	p-value (T-test)
IL6	Interleukin 6	↑	282.33	< 0.001	↓	14.36	< 0.001	Acute & Chronic Inflammation; Innate Immune Response
CFB	Complement factor B	↑	266.74	< 0.001	↓	37.74	< 0.001	Acute Inflammation; Innate Immune Response
C3	Complement component 3	↑	12.41	< 0.001	↓	3.63	< 0.001	Acute Inflammation; Innate & Adaptive Immune Response
CCL5	Chemokine (C–C motif) ligand 5	↑	402.43	< 0.001	↓	7.22	< 0.001	Chemokine; Acute Inflammation; Immunoregulatory and Inflammatory process
CXCL3	Chemokine (C-X-C motif) ligand 3	↑	11.17	0.011	↓	8.12	0.013	Chemokine; Chemoattractant; Acute Inflammation
1L12B	Interleukin 12B	↑	5.36	< 0.001	↓	4.47	< 0.001	Cytokine; Innate & Adaptive Immune Response; Apoptosis
IRF1	Interferon regulatory factor 1	↑	6.36	0.007	↓	5.03	0.008	Type I Interferon-Responsive Genes; Transcription Factor
Gene symbol	Encoded protein	Genes upregulated in the TQ-treated vs. control						Function
		Direction	Fold Regulation TQ/control	p-value (T-test)
NQO1	NAD(P)H: Quinone Oxidoredutase	↑	10.62	0.021				Antioxidant & Detoxification