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. Author manuscript; available in PMC: 2018 May 24.
Published in final edited form as: J Control Release. 2018 Feb 26;276:1–16. doi: 10.1016/j.jconrel.2018.02.033

Fig. 11.

Fig. 11

Th1 cytokine responses to viral infection were increased in GM-CSF-adjuvanted MN vaccinated mice. Mice vaccinated with A/Christchurch/16/2010 MN were infected with 10xLD50 mouse-adapted A/California/07/2009 virus 30 d.p.v. (A) Viral load (p.f.u.) was measured in lung lysates 4 days post-infection. Cytokine expression in lung lysates was quantified in a Bio-plex Pro Mouse Cytokine 8-plex assay. (B) IL-4 (C) IL-2 and (D) GM-CSF levels. Values are expressed as mean ± SEM. Analysis of Mann-Whitney test between 1 μg HA and 1 μg HA/100 ng GM-CSF reached statistical significance of p < 0.5 in ELISPOT assays for IL-4 and GM-CSF secretion.