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. Author manuscript; available in PMC: 2018 May 24.
Published in final edited form as: J Control Release. 2018 Feb 26;276:1–16. doi: 10.1016/j.jconrel.2018.02.033

Fig. 9.

Fig. 9

MN patch vaccination with GM-CSF increased CD8+ T cell responses in inguinal lymph nodes (ILN) and vaccine-specific IFN-γ responses in the spleen. Inguinal lymph nodes and spleens were harvested from mice 7 and 14 d.p.v. and measured for activation and vaccine-specific cytokine secretion. CD8+ follicular helper cells (CD8+CDXCR5+PD-1+) were determined for each sample, and representative images for (A) placebo, (B) 1 μg HA, (C) 1 μg HA, and (D) 1 μg HA are shown. (E) Frequency of CD8+ follicular helper cells in ILN were quantified in FlowJo and plotted in GraphPad Prism 7. (E) Vaccine-specific IFN-γ were isolated from the spleen at day 7 and enumerated in ELISPOT plates following stimulation with A/Christchurch/16/2010 subunit vaccine. Values are expressed as mean ± SEM and statistics were performed with Mann-Whitney test.