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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Mol Cancer Res. 2018 Feb 16;16(5):869–879. doi: 10.1158/1541-7786.MCR-17-0508

Figure 2.

Figure 2

CSC/EMT markers are elevated in tumors from DIO mice in association with increased local leptin signaling. (a) Tumor expression of several CSC/EMT-related genes in DIO and control mice identified by RNA sequencing analysis was verified via quantitative RT-PCR. (b) Tumor expression in DIO and control mice of previously established CSC/EMT-related genes (11) not identified by RNA sequencing was measured by quantitative RT-PCR. (c) ALDH activity, another marker of CSC enrichment, was quantified in DIO and control mouse tumors. (d) Immunohistochemical staining for tumor E-cadherin and vimentin expression in DIO and control mice. Representative images shown at x20 magnification. (e) Tumor expression of Lepr as well as mammary fat pad (MFP) expression of Lep in DIO and control mice were measured via quantitative RT-PCR. *P<0.05, **P<0.01