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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Mol Cancer Res. 2018 Feb 16;16(5):869–879. doi: 10.1158/1541-7786.MCR-17-0508

Figure 5.

Figure 5

Leptin contributes to an obesity-associated metastatic phenotype and CSC/EMT genotype. (a) Cell viability in parental E-Wnt cells (EWnt-P) as well as E-Wnt cells stably transfected with a scrambled shRNA plasmid (EWnt-S) or shRNA to Lepr (EWnt-L1 and EWnt-L2) was measured by MTT assay following a 48-hour exposure to media containing 2% DIO or control mouse serum. (b) Migration of EWnt-P, EWnt-S, EWnt-L1, and EWnt-L2 cells during a 6-hour exposure to media containing 2% DIO or control mouse serum was measured by wound healing assay. (c) The invasive capacity of EWnt-P, EWnt-S, EWnt-L1, and EWnt-L2 cells in response to chemoattraction with media containing 2% DIO or control mouse serum over 24 hours was measured using Matrigel invasion chambers. (d) Expression of CSC/EMT-related genes in EWnt-S and EWnt-L2 cells following a 24-hour exposure to media containing 2% DIO or control mouse serum was measured by quantitative RT-PCR. Different letters indicate significant differences, P<0.05.