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. 2018 May 24;13(5):e0197936. doi: 10.1371/journal.pone.0197936

Fig 1. LDHA is increased in bleomycin-induced pulmonary fibrosis.

Fig 1

Mice were exposed to saline or bleomycin via oropharyngeal aspiration (OA) and euthanized at indicated time points. (A) Total RNA was isolated from the right lung lobes and qRT-PCR was performed to measure LDHA mRNA. Data are displayed as fold change from saline controls normalized to 18S. *p≤0.05 compared to saline controls by t-test. n = 4–5 per group. (B) LDH activity was measured on lung tissue homogenates in mice administered either saline or bleomycin. ***p<0.001. (C-D) Lung tissue from mice at day 21 post bleomycin was stained for LDHA by immuno-histochemistry in red. Images were taken at 20X magnification, scale bars represent 50 μm. (E-F) Serial section immunohistochemistry was performed for LDH shown in panel E and αSMA shown in panel F. Images were taken at 40X magnification. LDHA expression localized to both macrophages (bold arrows) and areas co-staining for αSMA (open arrows), a marker of myofibroblast differentiation.