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. 2018 May 24;13(5):e0197936. doi: 10.1371/journal.pone.0197936

Fig 4. Gossypol prevents TGF-β1 induced fibronectin expression.

Fig 4

Mice were exposed to bleomycin and treated with gossypol as indicated in Fig 2 and sacrificed on day 21. Fibronectin expression was measured in whole lung homogenates by Western blot (A) which was quantified using densitometry (B). Data are displayed as mean ± SEM. **p<0.01 by ANOVA. n = 8 mice per treatment group. (C-F) Lung tissue sections were stained for fibronectin in red and counterstained with hematoxylin. (C) Saline + vehicle, (D) saline + gossypol 20mg/kg, (E) Bleomycin + vehicle at (F), bleomycin + gossypol 20mg/kg. All images were taken at 20X magnification (G). Scale bars represent 50 μm. The inset in (C) is an isotype negative control stain.