Figure 6.

Podocyte FPE is reduced in hLM-521–treated Lamb2−/− mice. Kidneys collected from P18 mice uninjected or injected with PBS or hLM-521 once daily for 5 days were analyzed by transmission electron microscopy. (A) The GBMs (asterisks in d–f) appeared normal, detected between the podocyte foot processes (arrows) and the endothelium (fenestrae marked with white arrowheads). Villous transformation in podocytes of Lamb2−/− mice (black arrowheads in e) was reduced (f) in Lamb2−/− mice treated with hLM-521. (B) When quantified by number of foot processes per micrometer of GBM, uninjected or PBS-injected Lamb2−/− mice showed significant reduction in the density of foot processes compared with Lamb2+/+ and Lamb2+/− controls (1.22±0.14, n=4 versus 2.18±0.07, n=3 glomeruli; ***P<0.001). hLM-521 treatment significantly increased the density of podocyte foot processes in Lamb2−/− mice (1.63±0.17, n=3; *P<0.05). FP, foot processes.