Figure 1.

Expression of PS is increased in diabetic rat glomeruli. (A) Real-time PCR was performed to measure Pros1 mRNA expression in glomeruli isolated from diabetic and control rats euthanized at either 6 or 12 weeks after the induction of diabetes (DM). Pros1 mRNA expression was also measured in diabetic rats treated with insulin between 6 and 12 weeks (DM+Insulin). (B) Representative images of immunostaining for PS are shown. Scale bar, 50 μm. (C) Glomerular region was selected, and OD was measured and quantified as a relative fold change to nondiabetic control mice (n=5; *P<0.05, **P<0.01, and ***P<0.001 when compared between indicated groups). AU, arbitrary units.