Figure 5.

Tyro3 mediates the effects of PS in podocytes. (A–C) Podocytes were incubated with normal glucose, high mannitol, or high glucose for 24 hours. Western blots were performed for each TAM receptor. (A) Representative blots of three independent experiments are shown. Densitometry analysis of Tyro3 is shown (densitometric analysis of Axl and Mer are included in Supplemental Figure 2). (C) PROS1 mRNA expression was measured by real-time PCR (mRNA expression of Axl and Mer are included in Supplemental Figure 2). (n=3; *P<0.05, **P<0.01, and ***P<0.001 versus Control.) (D and E) Podocytes stably transduced with lentivirus expressing either scrambled shRNA (shScr), Tyro3 shRNA (shTyro3), Axl shRNA (shAxl), or Mer ShRNA (ShMer) were transfected with NF-κB luciferase reporter and renilla luciferase, together with either control GFP overexpression vector (GFP^OE) or PS overexpression vector (PS^OE). (D) NF-κB luciferase reporter activity is shown as fold change to renilla luciferase activity. (E) Real-time PCR analysis of selected NF-κB–targeted gene expression was performed in these cells. (n=3; *P<0.05, **P<0.01, and ***P<0.001 when compared between indicated groups.)