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. 2018 May 14;12(5):e0006485. doi: 10.1371/journal.pntd.0006485

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© 2018 Sun et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — (A) SDS-PAGE analysis of ML ES antigens from T. spiralis (lane 1), ML crude antigen (lane 2), and rTsSP (lane 3). (B) Western blotting of the rTsSP. T. spiralis ML ES antigens (lane 1) and ML crude antigens (lane 2) and rTsSP (lane 3) were probed with mouse infection sera. The natural TsSP protein in ML ES (lane 4), ML crude antigens (lane 5) and rTsSP (lane 6) were identified by using anti-rTsSP serum. Normal mouse sera did not probe the ES (lane7) and crude antigens (lane 8), and rTsSP (lane 9). (C) Western blotting of ML ES (lane 1 and 4) and crude antigens (lane 2 and 5), and rTsSP (lane 3 and 6) probed with immune sera from mice vaccinated with ES and crude antigens.