Table 1. Thermodynamic stability of TBA variants modified with RNA-iG (iGR) or UNA-iG (iGU)a.
| Position of modification |
Sequence (5ʹ-3ʹ) |
Average of curve fits | |||||
|---|---|---|---|---|---|---|---|
| ΔG˚37 (kcal/mol) | TM (˚C) |
ΔΔG˚37 (kcal/mol) | ΔTM (˚C) | ΔΔG˚37 (kcal/mol) | ΔTM (˚C) | ||
| GGTTGGTGTGGTTGG | -1.74±0.02 | 50.7 | 0 | 0 | |||
| G1 | iGRGTTGGTGTGGTTGG | 1.49±0.48 | 19.9 | 3.23 | -30.8 | 0 | 0 |
| G1 | iGUGTTGGTGTGGTTGG | 1.55±0.07 | 20.7 | 3.29 | -30.0 | 0.06 | 0.8 |
| G8 | GGTTGGTiGRTGGTTGG | -0.51±0.02 | 41.6 | 1.23 | -9.1 | 0 | 0 |
| G8 | GGTTGGTiGUTGGTTGG | 0.21±0.01 | 35.1 | 1.95 | -15.6 | 0.72 | -6.5 |
| G10 | GGTTGGTGTiGRGTTGG | 0.39±0.05 | 31.9 | 2.13 | -18.8 | 0 | 0 |
| G10 | GGTTGGTGTiGUGTTGG | 1.65±0.33 | 23.8 | 3.39 | -26.9 | 1.26 | -8.1 |
| G1, G8 | iGRGTTGGTiGRTGGTTGG | 1.36±0.14 | 22.3 | 3.10 | -28.4 | ||
| G1, G8 | iGUGTTGGTiGUTGGTTGG | n.d. | <15.0 | - | - | - | - |
| G8, G10 | GGTTGGTiGRTiGRGTTGG | 0.31±0.01 | 34.1 | 2.05 | -16.6 | ||
| G8, G10 | GGTTGGTiGUTiGUGTTGG | n.d. | <15.0 | - | - | - | - |
| G1, G10 | iGRGTTGGTGTiGRGTTGG | n.d. | <15.0 | - | - | - | - |
| G1, G10 | iGUGTTGGTGTiGUGTTGG | n.d. | <15.0 | - | - | - | - |
| G1, G8, G10 | iGRGTTGGTiGRTiGRGTTGG | n.d. | <15.0 | - | - | - | - |
| G1, G8, G10 | iGUGTTGGTiGUTiGUGTTGG | n.d. | <15.0 | - | - | - | - |
a buffer: 100 mM KCl, 20 mM sodium cacodylate, 0.5 mM EDTA(Na)2, pH 7.0
n.d.–not determined, detailed thermodynamic analysis is presented in S3 Table