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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: IEEE Trans Biomed Eng. 2018 Jan;65(1):232–238. doi: 10.1109/TBME.2017.2773540

Fig. 3.

Fig. 3

Ex vivo comparison between (a) 2P-LSM and (b) resonant 2P-SuPER imaging a eGFP-positive neuron at 200 µm in an acute brain slice at 3.5 Hz. The corresponding magnified section of the (c) 2P-LSM (green box in panels a and d) resonant 2P-SuPER (blue box in b) images show that resonant 2P-SuPER maintains super-resolution in deep tissue and is more resilient to scattering than array based 2P-LSM. Comparisons between (e) 2P-LSM and (f) resonant 2P-SuPER imaging a eGFP neurons at 210 µm in the acute brain slice at 3.5 Hz showed an analogous resolution improvement. A magnified section of the (g) 2P-LSM (green box in panels e) and (h) resonant 2P-SuPER (blue box in panel f) images show that resonant 2P-SuPER maintained super-resolution at depths up to 210 µm from the cortical surface. Scale bars: 1 µm in (a) and (e), 500 nm in (c) and (g).